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Validating DNA Extraction Protocols for Bentonite Clay ...

2019-10-30  Bentonite clay is an integral component of the engineered barrier system of deep geological repositories (DGRs) that are planned for the long-term storage of high-level radioactive waste. Although nucleic acid extraction and analysis can provide powerful qualitative and quantitative data reflecting the presence, abundance, and functional potential of microorganisms within DGR materials ...

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Validating DNA Extraction Protocols for Bentonite Clay

IMPORTANCE Extraction of microbial DNA from MX-80 bentonite is challenging due to low biomass and adsorption of nucleic acid molecules to the charged clay matrix. Blocking agents improve DNA recovery, but their impact on microbial community profiles from low-biomass samples has not been characterized well. In this study, we evaluated the effect ...

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Validating DNA Extraction Protocols for Bentonite Clay.

2019-10-30  Validating DNA Extraction Protocols for Bentonite Clay. Bentonite clay is an integral component of the engineered barrier system of deep geological repositories (DGRs) that are planned for the long-term storage of high-level radioactive waste. Although nucleic acid extraction and analysis can provide powerful qualitative and quantitative data ...

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Validating DNA Extraction Protocols for Bentonite Clay ...

2019-10-30  DNA was extracted from all samples using the PowerSoil DNA isolation kit, except one sample of natural MX-80 bentonite was also extracted with the PowerMax DNA isolation kit (indicated by #). Evaluation of reagent and laboratory contaminants.

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Powersoil Dna Isolation Kit Qiagen Bioz

DNA was extracted from all samples using the PowerSoil DNA isolation kit, except one sample of natural MX-80 bentonite was also extracted with the PowerMax DNA isolation kit (indicated by #). Techniques Used: Denaturing Gradient Gel Electrophoresis, High Throughput Screening Assay, Amplification, Sequencing, Adsorption, DNA Extraction

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Microbiology of Bentonite Clay - UWSpace Home

The microbial communities contained within bentonite clay have traditionally been studied using culture-dependent detection methods. The difficulty associated with adsorption of DNA to the clay matrix has resulted in relatively few reports involving extraction and high-throughput sequencing of nucleic acids directly from mined bentonite clay ...

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Interaction of marine sediment with DNA and DNA ...

A method for the determination of DNA in sediments is described. The comparison between DNA extraction profiles from natural sediment samples and from precleaned sediments (concentrated nitric acid and subsequently 1 100 °C), to which known amounts of DNA were added, demonstrates that the sand particles participate in the delay of extraction of DNA we observed. Experiments with nuclease ...

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DNA extraction from Fungi - Fungal Genetics Stock Center

2014-10-15  Numerous reports have described procedures for the extraction and purification of fungal DNA. Many of these are modifications of the CTAB method originally developed for plant tissue extraction (Saghai-Maroof, et. al.1984 Proc. Natl. Acad. Sci. USA 81:8014-8018) or employ direct sample extraction with organic solvent as the principal means of denaturing and eliminating contaminating protein ...

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(PDF) EXTRACTION OF DNA FROM CHICKEN LIVER

PROCEDURE: 1. Weigh out 5g of chicken liver. 2. Drop the piece of tissue at a time into a chilled blender with. 150ml of cold saline citrate buffer. Continue to homogenise it for. about 30-60 secs ...

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Evaluation of methods for DNA extraction from must

DNA (RAPD) and microsatellites (SSR). The first step in all of these methods is the extraction of DNA from the test material, thus DNA preparation is a crucial point in the assessment. In order to apply the molecular marker methods, isolation of DNA in the appropriate amount and quality is required; however, DNA of sufficient quality and quan-

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Powersoil Dna Isolation Kit Qiagen Bioz

DNA was extracted from all samples using the PowerSoil DNA isolation kit, except one sample of natural MX-80 bentonite was also extracted with the PowerMax DNA isolation kit (indicated by #). Techniques Used: Denaturing Gradient Gel Electrophoresis, High Throughput Screening Assay, Amplification, Sequencing, Adsorption, DNA Extraction

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Bentonite - MP Bio

From complete isolation kits that simplify your workflows to individual reagents, we make it easy to get high-quality DNA and RNA from even difficult-to-lyse samples. DSS for Colitis and IBD Research Quickly generate murine models of intestinal inflammatory diseases using our

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Interaction of marine sediment with DNA and DNA ...

A method for the determination of DNA in sediments is described. The comparison between DNA extraction profiles from natural sediment samples and from precleaned sediments (concentrated nitric acid and subsequently 1 100 °C), to which known amounts of DNA were added, demonstrates that the sand particles participate in the delay of extraction of DNA we observed. Experiments with nuclease ...

Get Price

Improved yield and accuracy for DNA extraction in ...

2019-5-24  Nevertheless, DNA extraction continues to be a large source of experimental variability in 16S rRNA sequence analysis and the most time-consuming step in a high-throughput pipeline . Although DNA extraction protocols have been published for low bacterial biomasses [10,11], they are hard to implement as part of a high-throughput pipeline ...

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DNA extraction from Fungi - Fungal Genetics Stock Center

2014-10-15  Numerous reports have described procedures for the extraction and purification of fungal DNA. Many of these are modifications of the CTAB method originally developed for plant tissue extraction (Saghai-Maroof, et. al.1984 Proc. Natl. Acad. Sci. USA 81:8014-8018) or employ direct sample extraction with organic solvent as the principal means of denaturing and eliminating contaminating protein ...

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DELIVERABLE D2.8 Long-term stability of bentonite in the ...

2018-9-20  The bentonite was studied as a slurry in which water, gases, nutrients and microorganisms were able to move freely at the temperature hospitable for microorganisms. The objective was, to find out if microorganisms and the metabolites they produce are able to change the bentonite structure and if these changes could be significant for the bentonite

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DNA extraction for streamlined metagenomics of diverse ...

2018-3-16  A major bottleneck for metagenomic sequencing is rapid and efficient DNA extraction. Here, we compare the extraction efficiencies of three magnetic bead−based platforms (KingFisher, epMotion, and Tecan) to a standardized column-based extraction platform across a

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Comparison of DNA Extraction and Purification Methods

There is an increased interest in the extraction of nucleic acids from various environmental samples, since only a minority of naturally occurring microbes can be cultured using standard techniques. Nucleic acids extraction and purification from soils are extremely challenging due to the low biomass, high organic contents and high variability of soil types.

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Evaluation of methods for DNA extraction from must and

DNA (RAPD) and microsatellites (SSR). The first step in all of these methods is the extraction of DNA from the test material, thus DNA preparation is a crucial point in the assessment. In order to apply the molecular marker methods, isolation of DNA in the appropriate amount and quality is required; however, DNA

Get Price

Evaluation of methods for DNA extraction from must and ...

2014-4-15  The success of the DNA extraction depended on the restriction and ease-of-use of enzymes such as polymerase and ligase. If the DNA was not pure enough, contaminants inhibited polymerase chain reaction (PCR) amplification 5. In the current study, different DNA extraction methods were used for the isolation of pure DNA.

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Bentonite - MP Bio

From complete isolation kits that simplify your workflows to individual reagents, we make it easy to get high-quality DNA and RNA from even difficult-to-lyse samples. DSS for Colitis and IBD Research Quickly generate murine models of intestinal inflammatory diseases using our high-quality, high-purity dextran sulfate sodium (DSS).

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[Comparison of different methods for isolating total RNA ...

Then the results of the extraction were compared and analyzed by electrophoresis detection and RT-PCR verification. RESULT: The total RNA extracted by bentonite method were clear and no dispersion, the integrity of the RNA was well, and there was no obvious contamination with DNA and other impurities, was suitable for RT-PCR test.

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Interaction of marine sediment with DNA and DNA ...

A method for the determination of DNA in sediments is described. The comparison between DNA extraction profiles from natural sediment samples and from precleaned sediments (concentrated nitric acid and subsequently 1 100 °C), to which known amounts of DNA were added, demonstrates that the sand particles participate in the delay of extraction of DNA we observed. Experiments with nuclease ...

Get Price

DNA extraction from Fungi - Fungal Genetics Stock Center

2014-10-15  Numerous reports have described procedures for the extraction and purification of fungal DNA. Many of these are modifications of the CTAB method originally developed for plant tissue extraction (Saghai-Maroof, et. al.1984 Proc. Natl. Acad. Sci. USA 81:8014-8018) or employ direct sample extraction with organic solvent as the principal means of denaturing and eliminating contaminating protein ...

Get Price

DELIVERABLE D2.8 Long-term stability of bentonite in

2018-9-20  The bentonite was studied as a slurry in which water, gases, nutrients and microorganisms were able to move freely at the temperature hospitable for microorganisms. The objective was, to find out if microorganisms and the metabolites they produce are able to change the bentonite structure and if these changes could be significant for the bentonite

Get Price

Evaluation of methods for DNA extraction from must

DNA (RAPD) and microsatellites (SSR). The first step in all of these methods is the extraction of DNA from the test material, thus DNA preparation is a crucial point in the assessment. In order to apply the molecular marker methods, isolation of DNA in the appropriate amount and quality is required; however, DNA of sufficient quality and quan-

Get Price

Improved yield and accuracy for DNA extraction in ...

2019-5-24  Nevertheless, DNA extraction continues to be a large source of experimental variability in 16S rRNA sequence analysis and the most time-consuming step in a high-throughput pipeline . Although DNA extraction protocols have been published for low bacterial biomasses [10,11], they are hard to implement as part of a high-throughput pipeline ...

Get Price

Comparison of DNA Extraction and Purification Methods

There is an increased interest in the extraction of nucleic acids from various environmental samples, since only a minority of naturally occurring microbes can be cultured using standard techniques. Nucleic acids extraction and purification from soils are extremely challenging due to the low biomass, high organic contents and high variability of soil types.

Get Price

Evaluation of methods for DNA extraction from must and ...

2014-4-15  In this study, there were some modifications to the volume and centrifugation speeds of methods 3–5. Method 1. The DNA extraction from the young leaf was performed using the method described by Lodhi et al. 8 and the protocols of Lefort et al. 9, incorporating some modifications.The young leaf (0.5 g) was quickly ground with liquid nitrogen using a sterilized mortar and pestle.

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ISOLASI DNA RNA

2011-10-25  Jenis (BJ) antara DNA (1.5 –1.7 g/ml) dengan RNA (1.7 –2 g/ml). Sesudah sentrifugasi, RNA berada pada pellet, protein berada di atas supernatan, DNA terlarut dalam supernatan. Pellet RNA selanjutnya dicuci untuk menghilangkan kontaminan protein dan

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